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The implementation of AIxURO™ at PathNet has delivered a comprehensive set of clinical and operational advantages by bridging the gap between digital pathology and automated analytical software. By integrating this tool into the daily workflow, the laboratory realized several key benefits:
Strategic Clinical Uses
- Cyto-Histo Correlation: The platform serves as a powerful additional tool for correlating cytology findings with surgical pathology results.
- Quality Control Review: AIxURO™ provides a robust QC layer, acting as atireless "second set of eyes" to confirm results and reduce human fatigue.
- Educational Advancement: The digitized data and statistical analysis offer a modernized platform for the training and education of cytologists, residents and fellows.
Operational and Patient Benefits
- Cost-Effectiveness: Patients benefit from a reduction in unnecessary and expensive ancillary testing, such as UroVysion FISH, as the AI's precision assists in increased diagnostic confidence.
- Workflow Efficiency: The laboratory observed an increase in both cytologist and pathologist efficiency, particularly through the use of superior digital imaging.
- Ergonomics and Reliability: Beyond technical accuracy, the transition to digital analysis improved the physical ergonomics for cytologists and ensured the consistent confirmation of negative cases.
PathNet’s success proves that the future of pathology lies in the synergy between human expertise and machine learning. By embedding AI within the existing digital workflow, PathNet has empowered its staff to work more efficiently and comfortably while setting a new global standard for patient care.
The laboratory conducted a comprehensive validation study involving 117 cases across a wide range of diagnostic categories (benign, AUC, HGUC). The results showed:
- High Concordance: A 93.7% concordance rate between manual microscopy and the AI-driven
- Statistical Robustness: High sensitivity (90.4%) and specificity (94.1%),demonstrating the AI's ability to accurately identify HGUC cases while maintaining low false-positive rates.evaluation.
CorePlus has demonstrated that private laboratories can lead the way in adopting advanced AI technology. By prioritizing patient care and investing in digital tools, the lab has achieved unprecedented efficiency and diagnostic precision.
The implementation of the AI-driven workflow resulted in significant improvements:
- Increased Speed: Pathologists reported a 30% to 50% increase in workflow speed, with the diagnosis of benign cases often reduced to mere seconds.
- Enhanced Sensitivity: The AI was slightly biased toward sensitivity, ensuring that atypical cells were prioritized for review.
- Objective Data: The system provides quantitative metrics for atypical and suspicious cells, removing subjectivity from the diagnostic process.
- Improved Efficiency: The reduction in screening time allowed the lab to free up capacity for growth without compromising accuracy.
Clinical use of AIxURO resulted in a 30–50% faster overall read time, improved sensitivity with moreobjective data for atypical cases, and a noticeably better user experience. The system is easy to use,avoids unnecessary features, integrates well with glass slides, and supports increased efficiency andgrowth in routine clinical practice.
- Development of an automated deep-learning AI model for circulating tumor cell (CTC) analysis and enumeration
- Fluorescent microscopy CTC images (CK+/CD45-/DAPI+) collected from blood samples of non-small cell lung carcinoma patient by CMx CTC capture platform
- AI model developed with active learning implemented to train after expert image annotation on 20 slides and validation with 4 extra images
- 18 new test images studied for performance
- AI model predicted 34% more total CTC than current methods (1775 vs 1328)
- AI model recovered 45% more total CTCs absent from original human annotation (2507 vs 1732 events)
- AI model produced 90% time savings over conventional methods of enumeration (< 20 min vs approximately 4 hours)
- The model correctly characterized features of circulating tumor microthrombi (CTM), including CTC clusters and CTC-associated immune cells
An AI model trained to detect and enumerate circulating tumor cells in nonsmall cell lung cancer patients outperformed semiautomated methods, with higher sensitivity and significantly reduced review time (less than 20 minutes) for CTC enumeration in lung cancer specimens.
- Development of a deep-learning based image analysis model for cell classification and enumeration in urine cytology
- De-identified whole slide images (WSI) digitized and “active learning” approach used to train the model
- 3 sub-images (3335 cells) annotated by 3 domain experts for initial training
- Cells classified into 7 categories: High grade urothelial carcinoma (HGUC), cluster HGUC, atypical neoplastic cell, atypical reactive cell, inflammatory cell, epithelial cell, and unidentified cell, with expert feedback to the model
- Pilot study after training involved 2 sub-images from 5 digital slides (10 total sub-images)
- Ai model successfully learned the morphologies of all 6 cell types and was able to quantify total cell counts in each class
An artificial intelligence model that enumerates and classifies abnormal urothelial cells may improve urine cytology throughput, accuracy and reproducibility.

